Aquaculture and mcr Colistin Resistance Determinants

We read with great interest the article in mBio regarding the discovery of a third colistin resistance determinant, mcr-3, in plasmid pWJ1 isolated from porcine Escherichia coli in China (1). This discovery and the subsequently reported discovery of a fourth colistin resistance determinant, mcr-4 (2), provide further support for our hypothesis that the mcr determinants (phosphoethanolamine transferases) may have primarily or simultaneously originated in aquatic environments as a result of aquacultural activities, including integrated aquaculture, that bring together aquatic and terrestrial bacteria (3). The over 45 million metric tons of fish, crustaceans, and mollusks produced by aquaculture in China in 2014 far surpass the nearly 15 million metric tons of these animals captured by Chinese fisheries (4). More than 50% of this aquacultural production is exported, and this fast-growing industry employs millions of people (4). We have previously suggested that some of the original events that generated plasmidmediated colistin resistance genes mcr-1 and mcr-2 may have been stimulated by the heavy use of colistin and other antimicrobials in this industry in China through its facilitation of the capture and dissemination of potential colistin resistance genes from aquatic bacteria (3). Consistent with our hypothesis, mcr-3 found in Escherichia coli encodes a protein that shows significant amino acid sequence identity with phosphoethanolamine transferases of Aeromonas salmonicida (84%), Aeromonas hydrophila (76%), and Aeromonas piscicola (77%), while mcr-4 found in Salmonella encodes a phosphoethanolamine transferase with a 99% amino acid sequence identity with Shewanella frigidimarina (2). The mcr-3-containing plasmid pWJ1 has a type 1 integron, and it and the mcr-4-harboring bacteria also contain other antimicrobial resistance determinants [floR, aac(6=)-Ib-cr, sul, aadA, and tetA], which both we and others have found to be present in aquatic bacteria related to aquaculture (5), lending further credence to our hypothesis. Aeromonas and Shewanella are pathogens of aquacultured fish and, like other fish pathogens, can be naturally resistant to colistin (see Table S1 in reference 1). That members of these genera are also human pathogens may be one of the elements that aids dissemination of resistance determinants originating in aquatic organisms to terrestrial pathogens through horizontal gene transfer steps that remain uncharacterized. While the shuttling of mcr-3 and mcr-4 determinants from aquatic bacteria to human pathogens appears to be carried out by plasmids, a conjugative plasmid of the IncHI2 compatibility group in the case of mcr-3 (1) and a potentially mobilizable and transformable plasmid of the ColE type in the case of mcr-4 (2), the relevant question is how mcr determinants carried on the chromosome in Aeromonas and Shewanella become plasmid bound and transferable. As we have previously suggested, recombination between chromosomal and plasmid DNA segments could be mediated by insertion sequences, ISCR sequences, transposons, bacteriophages, and the insertion Published 3 October 2017 Citation Cabello FC, Tomova A, Ivanova L, Godfrey HP. 2017. Aquaculture andmcr colistin resistance determinants. mBio 8:e01229-17. https://doi.org/10.1128/mBio.01229-17. Editor Karen Bush, Indiana University Bloomington Copyright © 2017 Cabello et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license. Address correspondence to Felipe C. Cabello, [email protected]. * Present address: Larisa Ivanova, Department of Pediatrics, New York Medical College, Valhalla, New York, USA. LETTER TO THE EDITOR